Annotated with DAS 1.0 Annotated with DAS Finds similar protein sequence using remote BLAST. Using IDs of sequences found loads annotation for DAS sources. Uses UniProt BLAST and DAS Sources. das_annotation Input sequence Input reference sequence Sequence Protein Output variations Output variations Sequence Protein '*.gb' DB Database against which the search is performed: UniProtKB or clusters of sequences with 100%, 90% or 50% identity. Choice uniprotkb uniprotkb uniprotkb_archaea uniprotkb_bacteria uniprotkb_eukaryota uniprotkb_arthropoda uniprotkb_fungi uniprotkb_human uniprotkb_mammals uniprotkb_nematoda uniprotkb_plants uniprotkb_rodents uniprotkb_vertebrates uniprotkb_viruses uniprotkb_pdb uniprotkb_complete_microbial_proteomes uniprotkb_swissprot UniRef100 UniRef90 UniRef50 uniparc " --db=" + str(value)) IDs number Use first IDs of similar sequences to load annotations Integer 5 " --r=" + str(value)) Identity Minimum identity of a BLAST result and an input sequence. Integer 90 " --i=" + str(value)) Filtering Low-complexity regions (e.g. stretches of cysteine in Q03751, or hydrophobic regions in membrane proteins) tend to produce spurious, insignificant matches with sequences in the database which have the same kind of low-complexity regions, but are unrelated biologically. Choice false false true mask " --f=" + str(value)) Matrix The matrix assigns a probability score for each position in an alignment. Choice "" "" blosum45 blosum62 blosum80 pam70 pam30 " --m=" + str(value)) Threshold The expectation value (E) threshold is a statistical measure of the number of expected matches in a random database. The lower the e-value, the more likely the match is to be significant. Choice 10 0.0001 0.001 0.01 0.1 1 10 100 1000 " --t=" + str(value)) Gapped This will allow gaps to be introduced in the sequences when the comparison is done. Choice true false true " --g=" + str(value)) Max result Limits the number of returned alignments. Choice 250 50 100 250 500 750 1000 " --h=" + str(value))