##fileformat=VCFv4.1
##samtoolsVersion=0.1.19-44428cd
##reference=file:///Users/oigl/Desktop/UGENE_NGS_Sample_Data/Variant_Analysis/Common/chr21.fa
##contig=<ID=NC_000021,length=46944323>
##bcftoolsCommand=view -vc -d 0 -i -1 -p 0.5 -t 0.001 -1 0 -U 0 -X 0.01 -g -P full -
##INFO=<ID=DP,Number=1,Type=Integer,Description="Raw read depth">
##INFO=<ID=DP4,Number=4,Type=Integer,Description="# high-quality ref-forward bases, ref-reverse, alt-forward and alt-reverse bases">
##INFO=<ID=MQ,Number=1,Type=Integer,Description="Root-mean-square mapping quality of covering reads">
##INFO=<ID=MQ0,Number=1,Type=Integer,Description="Number of reads with zero mapping quality">
##INFO=<ID=FQ,Number=1,Type=Float,Description="Phred probability of all samples being the same">
##INFO=<ID=AF1,Number=1,Type=Float,Description="Max-likelihood estimate of the first ALT allele frequency (assuming HWE)">
##INFO=<ID=AC1,Number=1,Type=Float,Description="Max-likelihood estimate of the first ALT allele count (no HWE assumption)">
##INFO=<ID=AN,Number=1,Type=Integer,Description="Total number of alleles in called genotypes">
##INFO=<ID=IDV,Number=1,Type=Integer,Description="Maximum number of reads supporting an indel">
##INFO=<ID=IMF,Number=1,Type=Float,Description="Maximum fraction of reads supporting an indel">
##INFO=<ID=AC,Number=A,Type=Integer,Description="Allele count in genotypes for each ALT allele, in the same order as listed">
##INFO=<ID=G3,Number=3,Type=Float,Description="ML estimate of genotype frequencies">
##INFO=<ID=HWE,Number=1,Type=Float,Description="Chi^2 based HWE test P-value based on G3">
##INFO=<ID=CLR,Number=1,Type=Integer,Description="Log ratio of genotype likelihoods with and without the constraint">
##INFO=<ID=UGT,Number=1,Type=String,Description="The most probable unconstrained genotype configuration in the trio">
##INFO=<ID=CGT,Number=1,Type=String,Description="The most probable constrained genotype configuration in the trio">
##INFO=<ID=PV4,Number=4,Type=Float,Description="P-values for strand bias, baseQ bias, mapQ bias and tail distance bias">
##INFO=<ID=INDEL,Number=0,Type=Flag,Description="Indicates that the variant is an INDEL.">
##INFO=<ID=PC2,Number=2,Type=Integer,Description="Phred probability of the nonRef allele frequency in group1 samples being larger (,smaller) than in group2.">
##INFO=<ID=PCHI2,Number=1,Type=Float,Description="Posterior weighted chi^2 P-value for testing the association between group1 and group2 samples.">
##INFO=<ID=QCHI2,Number=1,Type=Integer,Description="Phred scaled PCHI2.">
##INFO=<ID=PR,Number=1,Type=Integer,Description="# permutations yielding a smaller PCHI2.">
##INFO=<ID=QBD,Number=1,Type=Float,Description="Quality by Depth: QUAL/#reads">
##INFO=<ID=SB,Number=1,Type=Float,Description="Strand Bias">
##INFO=<ID=EDB,Number=1,Type=Float,Description="End Distance Bias (tt2)">
##INFO=<ID=MQB,Number=1,Type=Float,Description="Mapping Quality Bias (tt2)">
##INFO=<ID=BQB,Number=1,Type=Float,Description="Base Quality Bias (tt2)">
##INFO=<ID=RPB,Number=1,Type=Float,Description="Read Position Bias">
##INFO=<ID=MDV,Number=1,Type=Integer,Description="Maximum number of high-quality nonRef reads in samples">
##INFO=<ID=VDB,Number=1,Type=Float,Description="Variant Distance Bias (v2) for filtering splice-site artefacts in RNA-seq data. Note: this version may be broken.">
##INFO=<ID=SGB,Number=1,Type=Float,Description="Segregation based metric.">
##FORMAT=<ID=GT,Number=1,Type=String,Description="Genotype">
##FORMAT=<ID=GQ,Number=1,Type=Integer,Description="Genotype Quality">
##FORMAT=<ID=GL,Number=3,Type=Float,Description="Likelihoods for RR,RA,AA genotypes (R=ref,A=alt)">
##FORMAT=<ID=DP,Number=1,Type=Integer,Description="# high-quality bases">
##FORMAT=<ID=DV,Number=1,Type=Integer,Description="# high-quality non-reference bases">
##FORMAT=<ID=SP,Number=1,Type=Integer,Description="Phred-scaled strand bias P-value">
##FORMAT=<ID=PL,Number=G,Type=Integer,Description="List of Phred-scaled genotype likelihoods">
#CHROM	POS	ID	REF	ALT	QUAL	FILTER	INFO	FORMAT	/Users/oigl/Desktop/UGENE_NGS_Sample_Data/Variant_Analysis/Call_Variants/reads.bam
NC_000021	9720141	.	C	T	37.8	.	DP=2;VDB=7.520000e-02;AF1=1;AC1=2;DP4=0,0,1,1;MQ=47;FQ=-33	GT:PL:GQ	1/1:69,6,0:10
NC_000021	9720865	.	C	T	32.8	.	DP=2;VDB=3.840000e-02;AF1=1;AC1=2;DP4=0,0,1,1;MQ=44;FQ=-33	GT:PL:GQ	1/1:64,6,0:10
NC_000021	9721015	.	A	C	38.8	.	DP=2;VDB=7.840000e-02;AF1=1;AC1=2;DP4=0,0,0,2;MQ=51;FQ=-33	GT:PL:GQ	1/1:70,6,0:10
NC_000021	9723074	.	A	T	46.8	.	DP=2;VDB=7.840000e-02;AF1=1;AC1=2;DP4=0,0,1,1;MQ=60;FQ=-33	GT:PL:GQ	1/1:78,6,0:10
NC_000021	9723372	.	G	C	30.8	.	DP=2;VDB=7.680000e-02;AF1=1;AC1=2;DP4=0,0,1,1;MQ=46;FQ=-33	GT:PL:GQ	1/1:62,6,0:10
NC_000021	9724114	.	T	G	31	.	DP=3;VDB=5.280000e-02;RPB=8.745357e-01;AF1=0.5002;AC1=1;DP4=0,1,1,1;MQ=58;FQ=4.77;PV4=1,0.23,0.33,0.33	GT:PL:GQ	0/1:61,0,30:33
NC_000021	9724168	.	T	C	22.8	.	DP=2;VDB=5.120000e-02;AF1=1;AC1=2;DP4=0,0,2,0;MQ=51;FQ=-33	GT:PL:GQ	1/1:54,6,0:10
NC_000021	9724177	.	C	A	18.8	.	DP=2;VDB=2.240000e-02;AF1=1;AC1=2;DP4=0,0,2,0;MQ=51;FQ=-33	GT:PL:GQ	1/1:50,6,0:10
NC_000021	9724295	.	T	C,G	30.8	.	DP=3;VDB=6.086650e-02;AF1=1;AC1=2;DP4=0,0,0,3;MQ=60;FQ=-33	GT:PL:GQ	1/1:89,33,27,61,0,58:10
NC_000021	9724307	.	A	T	60	.	DP=3;VDB=6.662997e-02;AF1=1;AC1=2;DP4=0,0,0,3;MQ=60;FQ=-36	GT:PL:GQ	1/1:92,9,0:16
NC_000021	9724343	.	T	C	34	.	DP=4;VDB=5.280000e-02;RPB=0.000000e+00;AF1=0.5;AC1=1;DP4=0,2,1,1;MQ=60;FQ=30;PV4=1,1,1,1	GT:PL:GQ	0/1:64,0,58:60
NC_000021	9724368	.	T	G	39	.	DP=3;VDB=4.320000e-02;RPB=8.745357e-01;AF1=0.5003;AC1=1;DP4=0,1,1,1;MQ=60;FQ=4.77;PV4=1,1,1,0.33	GT:PL:GQ	0/1:69,0,30:33
NC_000021	9724374	.	C	G	34	.	DP=3;VDB=4.320000e-02;RPB=8.745357e-01;AF1=0.5003;AC1=1;DP4=0,1,1,1;MQ=60;FQ=4.77;PV4=1,0.11,1,0.33	GT:PL:GQ	0/1:64,0,30:33
NC_000021	9724397	.	G	C	59	.	DP=3;VDB=6.086650e-02;AF1=1;AC1=2;DP4=0,0,1,2;MQ=50;FQ=-36	GT:PL:GQ	1/1:91,9,0:16
NC_000021	9724449	.	C	G	20.8	.	DP=2;VDB=6.400000e-02;AF1=1;AC1=2;DP4=0,0,0,2;MQ=44;FQ=-33	GT:PL:GQ	1/1:52,6,0:10
NC_000021	9724477	.	T	C,A	6.79	.	DP=3;VDB=1.420401e-02;AF1=1;AC1=2;DP4=0,0,1,2;MQ=42;FQ=-33	GT:PL:GQ	1/1:67,36,30,36,0,33:7
NC_000021	9724510	.	T	C	44.8	.	DP=3;VDB=4.800000e-02;AF1=1;AC1=2;DP4=0,0,1,1;MQ=51;FQ=-33	GT:PL:GQ	1/1:76,6,0:10
NC_000021	9724528	.	A	G	43.8	.	DP=2;VDB=3.360000e-02;AF1=1;AC1=2;DP4=0,0,1,1;MQ=41;FQ=-33	GT:PL:GQ	1/1:75,6,0:10
NC_000021	9724533	.	C	T	43.8	.	DP=2;VDB=4.960000e-02;AF1=1;AC1=2;DP4=0,0,1,1;MQ=41;FQ=-33	GT:PL:GQ	1/1:75,6,0:10
NC_000021	9724548	.	A	C	37	.	DP=3;VDB=6.240000e-02;RPB=8.745357e-01;AF1=0.501;AC1=1;DP4=1,0,1,1;MQ=48;FQ=-4.75;PV4=1,1,0.042,1	GT:PL:GQ	0/1:67,0,24:27
NC_000021	9724555	.	A	G	37	.	DP=3;VDB=4.000000e-02;RPB=8.745357e-01;AF1=0.5003;AC1=1;DP4=1,0,1,1;MQ=48;FQ=4.13;PV4=1,1,0.042,1	GT:PL:GQ	0/1:67,0,29:32
NC_000021	9724568	.	T	C	39	.	DP=3;VDB=2.400000e-02;RPB=8.745357e-01;AF1=0.5003;AC1=1;DP4=1,0,1,1;MQ=48;FQ=4.13;PV4=1,1,0.042,0.33	GT:PL:GQ	0/1:69,0,29:32
NC_000021	9724603	.	G	A	38.1	.	DP=3;VDB=4.800000e-02;RPB=8.745357e-01;AF1=0.505;AC1=1;DP4=1,0,1,1;MQ=55;FQ=-10.4;PV4=1,1,0.33,0.33	GT:PL:GQ	0/1:68,0,17:20
NC_000021	9724701	.	T	C	15.1	.	DP=3;VDB=7.040000e-02;RPB=8.745357e-01;AF1=0.5003;AC1=1;DP4=0,1,1,1;MQ=50;FQ=4.07;PV4=1,0.27,0.33,0.11	GT:PL:GQ	0/1:45,0,29:32
NC_000021	9724747	.	T	G	21.8	.	DP=2;VDB=2.720000e-02;AF1=1;AC1=2;DP4=0,0,0,2;MQ=44;FQ=-33	GT:PL:GQ	1/1:53,6,0:10
NC_000021	9726763	.	T	C	35.8	.	DP=2;VDB=6.400000e-02;AF1=1;AC1=2;DP4=0,0,2,0;MQ=60;FQ=-33	GT:PL:GQ	1/1:67,6,0:10
NC_000021	9731400	.	G	A	38.8	.	DP=2;VDB=6.560000e-02;AF1=1;AC1=2;DP4=0,0,2,0;MQ=60;FQ=-33	GT:PL:GQ	1/1:70,6,0:10
NC_000021	9733295	.	A	C	36.8	.	DP=2;VDB=6.560000e-02;AF1=1;AC1=2;DP4=0,0,1,1;MQ=60;FQ=-33	GT:PL:GQ	1/1:68,6,0:10
NC_000021	9733448	.	T	A	24.8	.	DP=2;VDB=5.280000e-02;AF1=1;AC1=2;DP4=0,0,2,0;MQ=45;FQ=-33	GT:PL:GQ	1/1:56,6,0:10
NC_000021	9735826	.	C	G	3.98	.	DP=2;VDB=5.960000e-02;AF1=1;AC1=2;DP4=0,0,2,0;MQ=60;FQ=-33	GT:PL:GQ	1/1:33,6,0:5
NC_000021	9736374	.	A	C	35.8	.	DP=2;VDB=4.000000e-02;AF1=1;AC1=2;DP4=0,0,1,1;MQ=60;FQ=-33	GT:PL:GQ	1/1:67,6,0:10
NC_000021	9736708	.	C	T	46.8	.	DP=2;VDB=7.680000e-02;AF1=1;AC1=2;DP4=0,0,1,1;MQ=60;FQ=-33	GT:PL:GQ	1/1:78,6,0:10
NC_000021	9736917	.	C	T	40.8	.	DP=2;VDB=3.520000e-02;AF1=1;AC1=2;DP4=0,0,1,1;MQ=60;FQ=-33	GT:PL:GQ	1/1:72,6,0:10
NC_000021	9737022	.	T	A	27.8	.	DP=2;VDB=5.120000e-02;AF1=1;AC1=2;DP4=0,0,2,0;MQ=60;FQ=-33	GT:PL:GQ	1/1:59,6,0:10
NC_000021	9737984	.	G	C	35.8	.	DP=2;VDB=4.160000e-02;AF1=1;AC1=2;DP4=0,0,0,2;MQ=60;FQ=-33	GT:PL:GQ	1/1:67,6,0:10
NC_000021	9738041	.	G	C,T	6.98	.	DP=2;VDB=2.400000e-02;AF1=1;AC1=2;DP4=0,0,1,1;MQ=60;FQ=-30	GT:PL:GQ	1/1:66,33,30,36,0,33:4
NC_000021	9738046	.	A	G	31.8	.	DP=2;VDB=1.120000e-02;AF1=1;AC1=2;DP4=0,0,1,1;MQ=60;FQ=-33	GT:PL:GQ	1/1:63,6,0:10
NC_000021	9743932	.	C	G	27.8	.	DP=2;VDB=5.120000e-02;AF1=1;AC1=2;DP4=0,0,1,1;MQ=57;FQ=-33	GT:PL:GQ	1/1:59,6,0:10
NC_000021	9803510	.	C	T,G	6.2	.	DP=2;VDB=4.800000e-02;AF1=1;AC1=2;DP4=0,0,1,1;MQ=44;FQ=-30	GT:PL:GQ	1/1:43,11,8,35,0,32:4
NC_000021	9912206	.	T	C	20.8	.	DP=2;VDB=2.400000e-02;AF1=1;AC1=2;DP4=0,0,1,1;MQ=60;FQ=-33	GT:PL:GQ	1/1:52,6,0:10
NC_000021	9912228	.	C	T	44.8	.	DP=2;VDB=6.560000e-02;AF1=1;AC1=2;DP4=0,0,1,1;MQ=60;FQ=-33	GT:PL:GQ	1/1:76,6,0:10
NC_000021	9912234	.	G	T	44.8	.	DP=2;VDB=4.640000e-02;AF1=1;AC1=2;DP4=0,0,1,1;MQ=60;FQ=-33	GT:PL:GQ	1/1:76,6,0:10
NC_000021	9912235	.	C	T	43.8	.	DP=2;VDB=4.320000e-02;AF1=1;AC1=2;DP4=0,0,1,1;MQ=60;FQ=-33	GT:PL:GQ	1/1:75,6,0:10
NC_000021	10134592	.	A	G	34.8	.	DP=2;VDB=5.960000e-02;AF1=1;AC1=2;DP4=0,0,1,1;MQ=60;FQ=-33	GT:PL:GQ	1/1:66,6,0:10
NC_000021	10142954	.	A	G	30.8	.	DP=2;VDB=7.520000e-02;AF1=1;AC1=2;DP4=0,0,2,0;MQ=60;FQ=-33	GT:PL:GQ	1/1:62,6,0:10
NC_000021	10149024	.	T	C	14.9	.	DP=2;VDB=3.840000e-02;AF1=1;AC1=2;DP4=0,0,2,0;MQ=38;FQ=-33	GT:PL:GQ	1/1:46,6,0:10
NC_000021	10152167	.	T	C	31.8	.	DP=2;VDB=5.280000e-02;AF1=1;AC1=2;DP4=0,0,1,1;MQ=60;FQ=-33	GT:PL:GQ	1/1:63,6,0:10
NC_000021	10164384	.	TTAGATAGATAG	TTAGATAGATAGATAG	53.2	.	INDEL;IS=1,0.500000;DP=2;VDB=6.240000e-02;AF1=1;AC1=2;DP4=0,0,1,1;MQ=54;FQ=-40.5	GT:PL:GQ	1/1:92,6,0:10
NC_000021	10164957	.	T	C	39.8	.	DP=2;VDB=6.240000e-02;AF1=1;AC1=2;DP4=0,0,2,0;MQ=60;FQ=-33	GT:PL:GQ	1/1:71,6,0:10
NC_000021	10165037	.	T	C	32.8	.	DP=2;VDB=4.320000e-02;AF1=1;AC1=2;DP4=0,0,2,0;MQ=60;FQ=-33	GT:PL:GQ	1/1:64,6,0:10
NC_000021	10168490	.	T	C	37.8	.	DP=2;VDB=7.040000e-02;AF1=1;AC1=2;DP4=0,0,0,2;MQ=60;FQ=-33	GT:PL:GQ	1/1:69,6,0:10
NC_000021	10168741	.	G	A	43.8	.	DP=2;VDB=6.720000e-02;AF1=1;AC1=2;DP4=0,0,1,1;MQ=60;FQ=-33	GT:PL:GQ	1/1:75,6,0:10
NC_000021	10170093	.	A	G	23.8	.	DP=2;VDB=5.960000e-02;AF1=1;AC1=2;DP4=0,0,0,2;MQ=60;FQ=-33	GT:PL:GQ	1/1:55,6,0:10
NC_000021	10172964	.	G	A	34.8	.	DP=2;VDB=6.240000e-02;AF1=1;AC1=2;DP4=0,0,0,2;MQ=60;FQ=-33	GT:PL:GQ	1/1:66,6,0:10
NC_000021	10208077	.	A	T	19.8	.	DP=2;VDB=2.240000e-02;AF1=1;AC1=2;DP4=0,0,1,1;MQ=26;FQ=-33	GT:PL:GQ	1/1:51,6,0:10
NC_000021	10208102	.	T	C	188	.	DP=14;VDB=6.861265e-02;AF1=1;AC1=2;DP4=0,0,12,1;MQ=53;FQ=-66	GT:PL:GQ	1/1:221,39,0:75
NC_000021	10208111	.	G	C	185	.	DP=17;VDB=1.036192e-01;AF1=1;AC1=2;DP4=0,0,16,1;MQ=47;FQ=-78	GT:PL:GQ	1/1:218,51,0:99
NC_000021	10208139	.	T	C	15.6	.	DP=4;VDB=2.064849e-02;AF1=1;AC1=2;DP4=0,0,4,0;MQ=16;FQ=-39	GT:PL:GQ	1/1:48,12,0:20
NC_000021	10208142	.	G	A	15.6	.	DP=4;VDB=2.064849e-02;AF1=1;AC1=2;DP4=0,0,4,0;MQ=16;FQ=-39	GT:PL:GQ	1/1:48,12,0:20
NC_000021	10208148	.	A	G	15.6	.	DP=4;VDB=2.064849e-02;AF1=1;AC1=2;DP4=0,0,4,0;MQ=16;FQ=-39	GT:PL:GQ	1/1:48,12,0:20
NC_000021	10208149	.	A	G	15.6	.	DP=4;VDB=2.064849e-02;AF1=1;AC1=2;DP4=0,0,4,0;MQ=16;FQ=-39	GT:PL:GQ	1/1:48,12,0:20
NC_000021	10208150	.	A	G	15.6	.	DP=4;VDB=2.064849e-02;AF1=1;AC1=2;DP4=0,0,4,0;MQ=16;FQ=-39	GT:PL:GQ	1/1:48,12,0:20
NC_000021	10208159	.	C	T	15.6	.	DP=4;VDB=2.064849e-02;AF1=1;AC1=2;DP4=0,0,4,0;MQ=16;FQ=-39	GT:PL:GQ	1/1:48,12,0:20
NC_000021	10208163	.	G	C	15.6	.	DP=4;VDB=2.064849e-02;AF1=1;AC1=2;DP4=0,0,4,0;MQ=16;FQ=-39	GT:PL:GQ	1/1:48,12,0:20
NC_000021	10208167	.	A	C	15.6	.	DP=4;VDB=2.064849e-02;AF1=1;AC1=2;DP4=0,0,4,0;MQ=16;FQ=-39	GT:PL:GQ	1/1:48,12,0:20
NC_000021	10208172	.	T	G	15.6	.	DP=4;VDB=2.064849e-02;AF1=1;AC1=2;DP4=0,0,4,0;MQ=16;FQ=-39	GT:PL:GQ	1/1:48,12,0:20
NC_000021	10208173	.	G	A	15.6	.	DP=4;VDB=2.064849e-02;AF1=1;AC1=2;DP4=0,0,4,0;MQ=16;FQ=-39	GT:PL:GQ	1/1:48,12,0:20
NC_000021	10208184	.	G	T	15.6	.	DP=4;VDB=2.064849e-02;AF1=1;AC1=2;DP4=0,0,4,0;MQ=16;FQ=-39	GT:PL:GQ	1/1:48,12,0:20
NC_000021	10208189	.	A	T	15.6	.	DP=4;VDB=2.064849e-02;AF1=1;AC1=2;DP4=0,0,4,0;MQ=16;FQ=-39	GT:PL:GQ	1/1:48,12,0:20
NC_000021	10208192	.	T	C	15.6	.	DP=4;VDB=2.064849e-02;AF1=1;AC1=2;DP4=0,0,4,0;MQ=16;FQ=-39	GT:PL:GQ	1/1:48,12,0:20
NC_000021	10208287	.	G	A	44	.	DP=4;VDB=3.131090e-02;RPB=-8.435450e-01;AF1=0.501;AC1=1;DP4=0,1,0,3;MQ=60;FQ=-4.75;PV4=1,0.39,1,0.028	GT:PL:GQ	0/1:74,0,24:27
NC_000021	10208301	.	G	T	91	.	DP=8;VDB=3.338131e-02;RPB=-6.573300e-01;AF1=0.5;AC1=1;DP4=0,2,0,6;MQ=60;FQ=21;PV4=1,0.17,1,1	GT:PL:GQ	0/1:121,0,48:51
NC_000021	10208310	.	G	C	113	.	DP=9;VDB=5.448794e-02;RPB=-1.936492e-01;AF1=0.5718;AC1=1;DP4=0,1,0,8;MQ=60;FQ=-21;PV4=1,1,1,0.39	GT:PL:GQ	0/1:142,0,6:9
NC_000021	10208312	.	T	A	107	.	DP=9;VDB=5.448794e-02;RPB=-1.936492e-01;AF1=0.5207;AC1=1;DP4=0,1,0,8;MQ=60;FQ=-16.1;PV4=1,0.23,1,0.34	GT:PL:GQ	0/1:137,0,11:14
NC_000021	10208315	.	T	C	115	.	DP=11;VDB=9.564745e-02;RPB=-3.481553e-01;AF1=0.5336;AC1=1;DP4=0,1,0,9;MQ=60;FQ=-18.1;PV4=1,0.18,1,0.25	GT:PL:GQ	0/1:145,0,9:12
NC_000021	10208325	.	T	C	167	.	DP=18;VDB=1.612395e-01;RPB=-9.637389e-01;AF1=1;AC1=2;DP4=0,1,1,16;MQ=60;FQ=-42;PV4=1,0.2,1,0.17	GT:PL:GQ	1/1:200,15,0:27
NC_000021	10208333	.	T	G	172	.	DP=18;VDB=1.774972e-01;RPB=-9.637389e-01;AF1=1;AC1=2;DP4=0,1,1,16;MQ=60;FQ=-42;PV4=1,0.21,1,0.26	GT:PL:GQ	1/1:205,15,0:27
NC_000021	10208334	.	A	G	198	.	DP=18;VDB=1.865132e-01;AF1=1;AC1=2;DP4=0,0,1,17;MQ=60;FQ=-81	GT:PL:GQ	1/1:231,54,0:99
NC_000021	10208345	.	T	A	175	.	DP=20;VDB=2.866004e-02;RPB=-1.004158e+00;AF1=1;AC1=2;DP4=0,1,1,17;MQ=60;FQ=-47;PV4=1,0.39,1,0.4	GT:PL:GQ	1/1:208,20,0:37
NC_000021	10208392	.	A	G	222	.	DP=29;VDB=5.450517e-02;AF1=1;AC1=2;DP4=0,0,15,13;MQ=60;FQ=-111	GT:PL:GQ	1/1:255,84,0:99
NC_000021	10208395	.	T	G	222	.	DP=28;VDB=2.249834e-01;RPB=-1.733156e+00;AF1=1;AC1=2;DP4=0,1,15,12;MQ=60;FQ=-72;PV4=0.46,0.2,1,1	GT:PL:GQ	1/1:255,45,0:87
NC_000021	10208422	.	A	G	222	.	DP=18;VDB=3.533507e-02;AF1=1;AC1=2;DP4=0,0,15,3;MQ=60;FQ=-81	GT:PL:GQ	1/1:255,54,0:99
NC_000021	10208424	.	A	T	222	.	DP=18;VDB=3.889063e-02;AF1=1;AC1=2;DP4=0,0,14,3;MQ=60;FQ=-78	GT:PL:GQ	1/1:255,51,0:99
NC_000021	10208427	.	C	T	222	.	DP=18;VDB=5.274041e-02;AF1=1;AC1=2;DP4=0,0,15,3;MQ=60;FQ=-81	GT:PL:GQ	1/1:255,54,0:99
NC_000021	10208429	.	T	C	222	.	DP=18;VDB=5.884374e-02;AF1=1;AC1=2;DP4=0,0,15,3;MQ=60;FQ=-81	GT:PL:GQ	1/1:255,54,0:99
NC_000021	10208441	.	T	G	219	.	DP=16;VDB=1.060382e-01;AF1=1;AC1=2;DP4=0,0,14,2;MQ=60;FQ=-75	GT:PL:GQ	1/1:252,48,0:93
NC_000021	10208470	.	C	T	114	.	DP=6;VDB=8.008355e-02;AF1=1;AC1=2;DP4=0,0,6,0;MQ=58;FQ=-45	GT:PL:GQ	1/1:147,18,0:33
NC_000021	10208518	.	T	A	111	.	DP=5;VDB=6.757191e-02;AF1=1;AC1=2;DP4=0,0,4,1;MQ=54;FQ=-42	GT:PL:GQ	1/1:144,15,0:27
NC_000021	10208521	.	C	T	100	.	DP=5;VDB=7.384173e-02;AF1=1;AC1=2;DP4=0,0,4,1;MQ=54;FQ=-42	GT:PL:GQ	1/1:133,15,0:27
NC_000021	10208531	.	A	G	116	.	DP=5;VDB=9.539213e-02;AF1=1;AC1=2;DP4=0,0,4,1;MQ=54;FQ=-42	GT:PL:GQ	1/1:149,15,0:27
NC_000021	10208537	.	A	G	115	.	DP=5;VDB=7.366457e-02;AF1=1;AC1=2;DP4=0,0,4,1;MQ=54;FQ=-42	GT:PL:GQ	1/1:148,15,0:27
NC_000021	10208552	.	G	A	48	.	DP=3;VDB=6.439972e-02;AF1=1;AC1=2;DP4=0,0,2,1;MQ=55;FQ=-36	GT:PL:GQ	1/1:80,9,0:16
NC_000021	10208585	.	G	A	121	.	DP=8;VDB=4.670117e-02;AF1=1;AC1=2;DP4=0,0,0,8;MQ=46;FQ=-51	GT:PL:GQ	1/1:154,24,0:45
NC_000021	10208587	.	C	G	116	.	DP=8;VDB=4.670117e-02;AF1=1;AC1=2;DP4=0,0,0,8;MQ=46;FQ=-51	GT:PL:GQ	1/1:149,24,0:45
NC_000021	10208593	.	T	A	131	.	DP=9;VDB=6.970820e-02;AF1=1;AC1=2;DP4=0,0,0,9;MQ=47;FQ=-54	GT:PL:GQ	1/1:164,27,0:51
NC_000021	10208603	.	C	A	137	.	DP=10;VDB=1.078812e-01;AF1=1;AC1=2;DP4=0,0,0,10;MQ=48;FQ=-57	GT:PL:GQ	1/1:170,30,0:57
NC_000021	10208605	.	A	C	135	.	DP=10;VDB=1.078812e-01;AF1=1;AC1=2;DP4=0,0,0,10;MQ=48;FQ=-57	GT:PL:GQ	1/1:168,30,0:57
NC_000021	10208618	.	C	T,A	141	.	DP=11;VDB=8.586036e-02;AF1=1;AC1=2;DP4=0,0,0,11;MQ=49;FQ=-57	GT:PL:GQ	1/1:174,30,0,160,6,157:57
NC_000021	10208623	.	G	A	148	.	DP=11;VDB=4.742703e-02;AF1=1;AC1=2;DP4=0,0,0,11;MQ=49;FQ=-60	GT:PL:GQ	1/1:181,33,0:63
NC_000021	10208639	.	C	T	153	.	DP=12;VDB=1.006690e-01;AF1=1;AC1=2;DP4=0,0,0,12;MQ=50;FQ=-63	GT:PL:GQ	1/1:186,36,0:69
NC_000021	10208682	.	C	G	36.8	.	DP=3;VDB=3.200000e-02;AF1=1;AC1=2;DP4=0,0,0,2;MQ=57;FQ=-33	GT:PL:GQ	1/1:68,6,0:10
NC_000021	13653915	.	C	T	15.2	.	DP=3;VDB=5.440000e-02;RPB=8.745357e-01;AF1=0.5032;AC1=1;DP4=1,0,2,0;MQ=60;FQ=-8.64;PV4=1,1,1,1	GT:PL:GQ	0/1:45,0,19:22
NC_000021	13653920	.	C	T	31	.	DP=3;VDB=5.440000e-02;RPB=8.745357e-01;AF1=0.5003;AC1=1;DP4=1,0,2,0;MQ=60;FQ=4.13;PV4=1,1,1,1	GT:PL:GQ	0/1:61,0,29:32
NC_000021	13653935	.	T	C	31	.	DP=3;VDB=5.440000e-02;RPB=8.745357e-01;AF1=0.5016;AC1=1;DP4=1,0,2,0;MQ=60;FQ=-6.18;PV4=1,1,1,0.33	GT:PL:GQ	0/1:61,0,22:25
NC_000021	13653939	.	T	C,A	3.59	.	DP=3;VDB=5.440000e-02;RPB=8.745357e-01;AF1=0.51;AC1=1;DP4=1,0,2,0;MQ=60;FQ=-13.3;PV4=1,1,1,1	GT:PL:GQ	0/1:45,14,28,17,0,31:21
NC_000021	13654450	.	A	G	37.8	.	DP=2;VDB=6.240000e-02;AF1=1;AC1=2;DP4=0,0,2,0;MQ=60;FQ=-33	GT:PL:GQ	1/1:69,6,0:10
NC_000021	13654502	.	G	C	39.8	.	DP=2;VDB=6.240000e-02;AF1=1;AC1=2;DP4=0,0,2,0;MQ=60;FQ=-33	GT:PL:GQ	1/1:71,6,0:10
NC_000021	13654504	.	A	G	38.8	.	DP=2;VDB=6.240000e-02;AF1=1;AC1=2;DP4=0,0,2,0;MQ=60;FQ=-33	GT:PL:GQ	1/1:70,6,0:10
NC_000021	14144733	.	T	C	33.8	.	DP=2;VDB=3.520000e-02;AF1=1;AC1=2;DP4=0,0,1,1;MQ=60;FQ=-33	GT:PL:GQ	1/1:65,6,0:10
NC_000021	14144751	.	C	T	42.8	.	DP=2;VDB=6.560000e-02;AF1=1;AC1=2;DP4=0,0,1,1;MQ=60;FQ=-33	GT:PL:GQ	1/1:74,6,0:10
NC_000021	14144805	.	G	A	30.8	.	DP=2;VDB=6.880000e-02;AF1=1;AC1=2;DP4=0,0,0,2;MQ=47;FQ=-33	GT:PL:GQ	1/1:62,6,0:10
NC_000021	14144810	.	A	C	22.8	.	DP=2;VDB=4.800000e-02;AF1=1;AC1=2;DP4=0,0,0,2;MQ=47;FQ=-33	GT:PL:GQ	1/1:54,6,0:10
NC_000021	14144814	.	A	G	30.8	.	DP=2;VDB=3.200000e-02;AF1=1;AC1=2;DP4=0,0,0,2;MQ=47;FQ=-33	GT:PL:GQ	1/1:62,6,0:10
NC_000021	14144815	.	A	G	28.8	.	DP=2;VDB=2.720000e-02;AF1=1;AC1=2;DP4=0,0,0,2;MQ=47;FQ=-33	GT:PL:GQ	1/1:60,6,0:10
NC_000021	14144818	.	C	T	30.8	.	DP=2;VDB=2.560000e-02;AF1=1;AC1=2;DP4=0,0,0,2;MQ=47;FQ=-33	GT:PL:GQ	1/1:62,6,0:10
NC_000021	14144827	.	C	T	16.9	.	DP=2;VDB=3.520000e-02;AF1=1;AC1=2;DP4=0,0,0,2;MQ=47;FQ=-33	GT:PL:GQ	1/1:48,6,0:10
NC_000021	14199566	.	A	G	19.8	.	DP=2;VDB=3.360000e-02;AF1=1;AC1=2;DP4=0,0,0,2;MQ=42;FQ=-33	GT:PL:GQ	1/1:51,6,0:10
NC_000021	14199576	.	T	A	14.9	.	DP=2;VDB=3.360000e-02;AF1=1;AC1=2;DP4=0,0,0,2;MQ=42;FQ=-33	GT:PL:GQ	1/1:46,6,0:10
NC_000021	14199581	.	C	T	13	.	DP=3;VDB=3.520000e-02;AF1=1;AC1=2;DP4=0,0,0,2;MQ=42;FQ=-33	GT:PL:GQ	1/1:44,6,0:9
NC_000021	14199601	.	A	G	56	.	DP=3;VDB=6.280960e-02;AF1=1;AC1=2;DP4=0,0,1,2;MQ=40;FQ=-36	GT:PL:GQ	1/1:88,9,0:16
NC_000021	14199610	.	A	G	51	.	DP=3;VDB=6.439972e-02;AF1=1;AC1=2;DP4=0,0,1,2;MQ=40;FQ=-36	GT:PL:GQ	1/1:83,9,0:16
NC_000021	14199613	.	C	T	56	.	DP=3;VDB=6.568498e-02;AF1=1;AC1=2;DP4=0,0,1,2;MQ=40;FQ=-36	GT:PL:GQ	1/1:88,9,0:16
NC_000021	14199628	.	G	C	24.8	.	DP=3;VDB=5.280000e-02;AF1=1;AC1=2;DP4=0,0,1,1;MQ=29;FQ=-33	GT:PL:GQ	1/1:56,6,0:10
NC_000021	14199636	.	G	T	24.8	.	DP=2;VDB=4.960000e-02;AF1=1;AC1=2;DP4=0,0,1,1;MQ=29;FQ=-33	GT:PL:GQ	1/1:56,6,0:10
NC_000021	14199641	.	C	T	24.8	.	DP=2;VDB=4.960000e-02;AF1=1;AC1=2;DP4=0,0,1,1;MQ=29;FQ=-33	GT:PL:GQ	1/1:56,6,0:10
NC_000021	14199644	.	A	C	24.8	.	DP=2;VDB=4.960000e-02;AF1=1;AC1=2;DP4=0,0,1,1;MQ=29;FQ=-33	GT:PL:GQ	1/1:56,6,0:10
NC_000021	14201437	.	A	G	10.2	.	DP=2;VDB=2.560000e-02;AF1=1;AC1=2;DP4=0,0,1,1;MQ=43;FQ=-33	GT:PL:GQ	1/1:41,6,0:8
NC_000021	15816053	.	A	T	13	.	DP=2;VDB=5.120000e-02;AF1=1;AC1=2;DP4=0,0,2,0;MQ=42;FQ=-33	GT:PL:GQ	1/1:44,6,0:9
NC_000021	15816058	.	T	C	13.9	.	DP=2;VDB=6.240000e-02;AF1=1;AC1=2;DP4=0,0,2,0;MQ=42;FQ=-33	GT:PL:GQ	1/1:45,6,0:10
NC_000021	22259080	.	C	T	33	.	DP=3;VDB=5.960000e-02;RPB=-8.745357e-01;AF1=0.5002;AC1=1;DP4=0,1,1,1;MQ=60;FQ=4.77;PV4=1,0.33,1,1	GT:PL:GQ	0/1:63,0,30:33
NC_000021	22259091	.	A	G	43.8	.	DP=2;VDB=5.960000e-02;AF1=1;AC1=2;DP4=0,0,1,1;MQ=60;FQ=-33	GT:PL:GQ	1/1:75,6,0:10
NC_000021	46210010	.	G	A	15.9	.	DP=2;VDB=7.520000e-02;AF1=1;AC1=2;DP4=0,0,1,1;MQ=27;FQ=-33	GT:PL:GQ	1/1:47,6,0:10
NC_000021	46210047	.	G	A	15.9	.	DP=2;VDB=5.600000e-02;AF1=1;AC1=2;DP4=0,0,1,1;MQ=27;FQ=-33	GT:PL:GQ	1/1:47,6,0:10