Sequence name	pr1_F pr1_R	pr2_F pr2_R	pr3_F pr3_R	pr4_F pr4_R	pr5_F pr5_R	pr6_F pr6_R	pr7_F pr7_R	pr8_F pr8_R	pr9_F pr9_R	pr10_F pr10_R	pr11_F pr11_R	pr12_F pr12_R	pr13_F pr13_R
LN866313	1	1	0	1	1	0	1	1	1	0	1	1	1

Criteria	Valid Values	Forward	Reverse
% GC	50-60	70.59	50
Tm (°C)	55-80	54.29	48.04
GC Clamp	>=1 G or C at 3' end	4	2
Runs	<=4 base runs	2	4

Self-dimers:

Delta G: -9.3 kcal/mole Base Pairs: 5

TCCAGGAGCGAGGCCAC
         : |||| :         
         CACCGGAGCGAGGACCT

Delta G: -9.3 kcal/mole Base Pairs: 5

      TGAAGGCCCCATTGAAAC
          ||||    
CAAAGTTACCCCGGAAGT

Hetero-dimers:

Delta G: -9.3 kcal/mole Base Pairs: 5

TCCAGGAGCGAGGCCAC
      :  : ||||   
 CAAAGTTACCCCGGAAGT

pr4_F / pr4_R

Criteria	Valid Values	Forward	Reverse
% GC	50-60	50	55
Tm (°C)	55-80	51.78	53.83
GC Clamp	>=1 G or C at 3' end	2	5
Runs	<=4 base runs	3	4

Self-dimers:

Delta G: -7.1 kcal/mole Base Pairs: 5

CGCAAGCGATTTGCAGACAA
         : |||| :         
      AACAGACGTTTAGCGAACGC

Hetero-dimers:

Delta G: -6.7 kcal/mole Base Pairs: 4

 CGCAAGCGATTTGCAGACAA
 |||              : 
GGCGGCTCTAGTTTTCAGGA

pr5_F / pr5_R

Criteria	Valid Values	Forward	Reverse
% GC	50-60	55	55
Tm (°C)	55-80	53.83	53.83
GC Clamp	>=1 G or C at 3' end	2	4
Runs	<=4 base runs	3	4

Self-dimers:

Delta G: -9.3 kcal/mole Base Pairs: 5

CAACACCCTTGGCCTCGATA
       :  ||||  :       
    ATAGCTCCGGTTCCCACAAC

Hetero-dimers:

Delta G: -6.2 kcal/mole Base Pairs: 4

CAACACCCTTGGCCTCGATA
       :  |||    ::        
       GGTCCGCCAGTACCTTTTCT

pr6_F / pr6_R

Criteria	Valid Values	Forward	Reverse
% GC	50-60	47.62	47.62
Tm (°C)	55-80	52.4	52.4
GC Clamp	>=1 G or C at 3' end	3	3
Runs	<=4 base runs	3	2

Self-dimers:

Delta G: -9.3 kcal/mole Base Pairs: 7

GACTGGTTCCAATTGACAAGC
      :  ||||||  :      
   CGAACAGTTAACCTTGGTCAG

pr7_F / pr7_R

Criteria	Valid Values	Forward	Reverse
% GC	50-60	55	55
Tm (°C)	55-80	53.83	53.83
GC Clamp	>=1 G or C at 3' end	2	2
Runs	<=4 base runs	4	3

Self-dimers:

Delta G: -8.2 kcal/mole Base Pairs: 5

  ATTGGGTGATGCCCCATTCC
  : ||||  ::  :::: :
CCTTACCCCGTAGTGGGTTA

Delta G: -9.3 kcal/mole Base Pairs: 5

    TATCGAGGCCAAGGGTGTTG
       :  ||||  :   
GTTGTGGGAACCGGAGCTAT

Hetero-dimers:

Delta G: -7 kcal/mole Base Pairs: 5

       ATTGGGTGATGCCCCATTCC
        ||||        
GTTGTGGGAACCGGAGCTAT

pr8_F / pr8_R

Criteria	Valid Values	Forward	Reverse
% GC	50-60	60	60
Tm (°C)	55-80	55.88	55.88
GC Clamp	>=1 G or C at 3' end	4	4
Runs	<=4 base runs	3	3

Self-dimers:

Delta G: -14.4 kcal/mole Base Pairs: 8

            CGATATCGAGGCCAAGGGTG
            ||||||||
GTGGGAACCGGAGCTATAGC

Hetero-dimers:

Delta G: -8.6 kcal/mole Base Pairs: 6

CCCATTCCTTGATCGGCTCC
      |||||  :         
   GTGGGAACCGGAGCTATAGC

pr9_F / pr9_R

Criteria	Valid Values	Forward	Reverse
% GC	50-60	60	50
Tm (°C)	55-80	55.88	51.78
GC Clamp	>=1 G or C at 3' end	4	3
Runs	<=4 base runs	4	2

Hetero-dimers:

Delta G: -6.3 kcal/mole Base Pairs: 5

CCCCATTCCTTGATCGGCTC
       :        ||||      
      TGACCAAGTACGAGTACGGA

pr10_F / pr10_R

Criteria	Valid Values	Forward	Reverse
% GC	50-60	37.5	50
Tm (°C)	55-80	52.26	51.78
GC Clamp	>=1 G or C at 3' end	2	3
Runs	<=4 base runs	3	4

Hetero-dimers:

Delta G: -6.2 kcal/mole Base Pairs: 6

CTACAAACTCTTCCTGTTAGTTAG
       :      |||||            
       GTTCCCCACAATACTCGGTA

pr11_F / pr11_R

Criteria	Valid Values	Forward	Reverse
% GC	50-60	50	50
Tm (°C)	55-80	51.78	51.78
GC Clamp	>=1 G or C at 3' end	3	2
Runs	<=4 base runs	3	3

Self-dimers:

Delta G: -6.7 kcal/mole Base Pairs: 4

TTTGGCATATCCGCAAGCGA
           |||  :::           
          AGCGAACGCCTATACGGTTT

Hetero-dimers:

Delta G: -7 kcal/mole Base Pairs: 5

             TTTGGCATATCCGCAAGCGA
              |||| :
TTGTCGGTGAGAACAACCCT

pr12_F / pr12_R

Criteria	Valid Values	Forward	Reverse
% GC	50-60	55	50
Tm (°C)	55-80	53.83	51.78
GC Clamp	>=1 G or C at 3' end	2	1
Runs	<=4 base runs	3	3

Self-dimers:

Delta G: -9.3 kcal/mole Base Pairs: 7

            CCAATTGCTCTCGCCACTTT
             |||||| 
TTTCACCGCTCTCGTTAACC

Hetero-dimers:

Delta G: -8.5 kcal/mole Base Pairs: 5

CACTCTTTGCGTGCGATTGG
 :      : : :: |||||
TTTCACCGCTCTCGTTAACC

pr13_F / pr13_R

Criteria	Valid Values	Forward	Reverse
% GC	50-60	55	50
Tm (°C)	55-80	53.83	51.78
GC Clamp	>=1 G or C at 3' end	2	2
Runs	<=4 base runs	2	3

Hetero-dimers:

Delta G: -8.3 kcal/mole Base Pairs: 5

 GAGGAATCCAGCGAGACACT
        :  ||||     
TGAGAAACGCACGCTAACCT

Products count table

Primer pair details

pr1_F / pr1_R

pr2_F / pr2_R

pr3_F / pr3_R

Self-dimers:

Hetero-dimers:

pr4_F / pr4_R

Self-dimers:

Hetero-dimers:

pr5_F / pr5_R

Self-dimers:

Hetero-dimers:

pr6_F / pr6_R

Self-dimers:

pr7_F / pr7_R

Self-dimers:

Hetero-dimers:

pr8_F / pr8_R

Self-dimers:

Hetero-dimers:

pr9_F / pr9_R

Hetero-dimers:

pr10_F / pr10_R

Hetero-dimers:

pr11_F / pr11_R

Self-dimers:

Hetero-dimers:

pr12_F / pr12_R

Self-dimers:

Hetero-dimers:

pr13_F / pr13_R

Hetero-dimers: