LOCUS       STEP1_pFUS2_a2a_5       2885 bp     circular            23-AUG-2018
UNIMARK     STEP1_pFUS2_a2a_5 annotations
            STEP1_pFUS2_a2a_5
FEATURES             Location/Qualifiers
     misc_feature    1..2477
                     /source_doc="pFUS2_a2a.gb"
                     /ugene_name="backbone_pFUS2_a2a"
                     /ugene_group="Exported\ Fragment\ 1"
     misc_feature    1..2885
                     /comment="Molecule is created with Unipro UGENE v1.31"
                     /ugene_name="source"
     misc_feature    complement(4..9)
                     /note="Esp3I site"
     misc_feature    108..127
                     /ugene_name="pCR8_R1"
     CDS             369..1160
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /note="SmR"
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1253..1841
                     /direction="RIGHT"
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      2171..2198
                     /note="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      2290..2376
                     /gene="Escherichia coli rrnB"
                     /note="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     misc_feature    2374..2393
                     /ugene_name="pCR8_F1"
     primer_bind     2440..2456
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    2463..2468
                     /note="Esp3I site"
     misc_feature    2482..2578
                     /source_doc="p1NN.gb"
                     /ugene_name="NN_repeat"
                     /ugene_group="Exported\ Fragment\ 2"
     misc_feature    2583..2679
                     /source_doc="p2NI.gb"
                     /ugene_name="NI_repeat"
                     /ugene_group="Exported\ Fragment\ 2"
     misc_feature    2684..2780
                     /source_doc="p3HD.gb"
                     /ugene_name="HD_repeat"
                     /ugene_group="Exported\ Fragment\ 2"
     misc_feature    2785..2881
                     /source_doc="p4NN.gb"
                     /ugene_name="NN_repeat"
                     /ugene_group="Exported\ Fragment\ 2"
ORIGIN
        1 ACCGAGACGT CTAGACCAGC CAGGACAGAA ATGCCTCGAC TTCGCTGCTA CCCAAGGTTG
       61 CCGGGTGACG CACACCGTGG AAACGGATGA AGGCACGAAC CCAGTGGACA TAAGCCTGTT
      121 CGGTTCGTAA GCTGTAATGC AAGTAGCGTA TGCGCTCACG CAACTGGTCC AGAACCTTGA
      181 CCGAACGCAG CGGTGGTAAC GGCGCAGTGG CGGTTTTCAT GGCTTGTTAT GACTGTTTTT
      241 TTGGGGTACA GTCTATGCCT CGGGCATCCA AGCAGCAAGC GCGTTACGCC GTGGGTCGAT
      301 GTTTGATGTT ATGGAGCAGC AACGATGTTA CGCAGCAGGG CAGTCGCCCT AAAACAAAGT
      361 TAAACATTAT GAGGGAAGCG GTGATCGCCG AAGTATCGAC TCAACTATCA GAGGTAGTTG
      421 GCGTCATCGA GCGCCATCTC GAACCGACGT TGCTGGCCGT ACATTTGTAC GGCTCCGCAG
      481 TGGATGGCGG CCTGAAGCCA CACAGTGATA TTGATTTGCT GGTTACGGTG ACCGTAAGGC
      541 TTGATGAAAC AACGCGGCGA GCTTTGATCA ACGACCTTTT GGAAACTTCG GCTTCCCCTG
      601 GAGAGAGCGA GATTCTCCGC GCTGTAGAAG TCACCATTGT TGTGCACGAC GACATCATTC
      661 CGTGGCGTTA TCCAGCTAAG CGCGAACTGC AATTTGGAGA ATGGCAGCGC AATGACATTC
      721 TTGCAGGTAT CTTCGAGCCA GCCACGATCG ACATTGATCT GGCTATCTTG CTGACAAAAG
      781 CAAGAGAACA TAGCGTTGCC TTGGTAGGTC CAGCGGCGGA GGAACTCTTT GATCCGGTTC
      841 CTGAACAGGA TCTATTTGAG GCGCTAAATG AAACCTTAAC GCTATGGAAC TCGCCGCCCG
      901 ACTGGGCTGG CGATGAGCGA AATGTAGTGC TTACGTTGTC CCGCATTTGG TACAGCGCAG
      961 TAACCGGCAA AATCGCGCCG AAGGATGTCG CTGCCGACTG GGCAATGGAG CGCCTGCCGG
     1021 CCCAGTATCA GCCCGTCATA CTTGAAGCTA GACAGGCTTA TCTTGGACAA GAAGAAGATC
     1081 GCTTGGCCTC GCGCGCAGAT CAGTTGGAAG AATTTGTCCA CTACGTGAAA GGCGAGATCA
     1141 CCAAGGTAGT CGGCAAATAA CCCTCGAGCC ACCCATGACC AAAATCCCTT AACGTGAGTT
     1201 ACGCGTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC
     1261 CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG
     1321 TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG
     1381 CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT
     1441 CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG
     1501 GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC
     1561 GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG
     1621 AACTGAGATA CCTACAGCGT GAGCATTGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG
     1681 CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG
     1741 GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC
     1801 GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT
     1861 TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC
     1921 CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC
     1981 GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCCA ATACGCAAAC
     2041 CGCCTCTCCC CGCGCGTTGG CCGATTCATT AATGCAGCTG GCACGACAGG TTTCCCGACT
     2101 GGAAAGCGGG CAGTGAGCGC AACGCAATTA ATACGCGTAC CGCTAGCCAG GAAGAGTTTG
     2161 TAGAAACGCA AAAAGGCCAT CCGTCAGGAT GGCCTTCTGC TTAGTTTGAT GCCTGGCAGT
     2221 TTATGGCGGG CGTCCTGCCC GCCACCCTCC GGGCCGTTGC TTCACAACGT TCAAATCCGC
     2281 TCCCGGCGGA TTTGTCCTAC TCAGGAGAGC GTTCACCGAC AAACAACAGA TAAAACGAAA
     2341 GGCCCAGTCT TCCGACTGAG CCTTTCGTTT TATTTGATGC CTGGCAGTTC CCTACTCTCG
     2401 CGTTAACGCT AGCATGGATG TTTTCCCAGT CACGACGTTG TAAAACGACG GCCAGTCTTA
     2461 AGCGTCTCAT CAACCTGACC CCAGACCAAG TTGTCGCGAT TGCAAGCAAC AACGGAGGCA
     2521 AACAAGCCTT AGAAACAGTC CAGAGATTGT TGCCGGTGCT GTGCCAAGAC CACGGCCTGA
     2581 CCCCAGAACA GGTTGTGGCC ATCGCCAGCA ACATAGGTGG CAAGCAGGCC CTCGAAACCG
     2641 TCCAGAGACT GTTACCGGTT CTCTGCCAGG CCCACGGCCT GACCCCGGAC CAGGTGGTTG
     2701 CAATCGCGTC ACACGATGGG GGAAAGCAGG CCCTAGAAAC CGTTCAGCGA CTCCTGCCCG
     2761 TCCTGTGCCA GGCCCACGGC CTGACCCCAG CCCAAGTTGT CGCGATTGCA AGCAACAACG
     2821 GAGGCAAACA AGCCTTAGAA ACAGTCCAGA GATTGTTGCC GGTGCTGTGC CAAGACCACG
     2881 GCCTG
//
