LOCUS ABCGTD010000000 23 rc DNA linear BCT 03-SEP-2021 DEFINITION Listeria monocytogenes strain PNUSAL011489, whole genome shotgun sequencing project. ACCESSION ABCGTD000000000 VERSION ABCGTD000000000.1 DBLINK BioProject: PRJNA212117 BioSample: SAMN21207824 Sequence Read Archive: SRR15701498 KEYWORDS WGS; GMI. SOURCE Listeria monocytogenes ORGANISM Listeria monocytogenes Bacteria; Firmicutes; Bacilli; Bacillales; Listeriaceae; Listeria. REFERENCE 1 (bases 1 to 23) CONSRTM PulseNet: The National Subtyping Network for Foodborne Disease Surveillance TITLE Direct Submission JOURNAL Submitted (02-SEP-2021) Enteric Diseases Laboratory Branch, Centers for Disease Control and Prevention, 1600 Clifton Rd., MS C03, Atlanta, GA 30333, USA COMMENT The Listeria monocytogenes whole genome shotgun (WGS) project has the project accession ABCGTD000000000. This version of the project (01) has the accession number ABCGTD010000000, and consists of sequences ABCGTD010000001-ABCGTD010000023. The annotation was added by the assembly submitters using the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about stand-alone PGAP can be found here: https://github.com/ncbi/pgap/ This draft WGS assembly was generated by running SKESA to generate a de-novo assembly. The de-novo assembly was then concatenated with contigs generated using a guided assembler using antimicrobial resistance genes as baits to comprehensively catalog the set of resistance genes in the isolate. Note, some parts of the contigs derived from the guided assembler may overlap de-novo contigs, and other guided assembler contigs. De-novo contigs can be differentiated from guided assembler contigs by their names , which include either 'denovo' or 'guided'. ##Genome-Assembly-Data-START## Assembly Date :: 02-SEP-2021 Assembly Method :: SKESA v. 2.2 Assembly Name :: PDT001122375.1 Long Assembly Name :: NCBI Pathogen Detection Assembly PDT001122375.1 Genome Coverage :: 81x Sequencing Technology :: ILLUMINA ##Genome-Assembly-Data-END## ##Genome-Annotation-Data-START## Annotation Date :: 09/02/2021 21:25:48 Annotation Method :: Best-placed reference protein set; GeneMarkS-2+ Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline (PGAP) Annotation Provider :: NCBI Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region Annotation Software revision :: 2021-01-11.build5132 Genes (total) :: 3,145 CDSs (total) :: 3,073 Genes (coding) :: 3,053 CDSs (with protein) :: 3,053 Genes (RNA) :: 72 rRNAs :: 2, 1, 2 (5S, 16S, 23S) complete rRNAs :: 2 (5S) partial rRNAs :: 1, 2 (16S, 23S) tRNAs :: 63 ncRNAs :: 4 Pseudo Genes (total) :: 20 CDSs (without protein) :: 20 Pseudo Genes (ambiguous residues) :: 0 of 20 Pseudo Genes (frameshifted) :: 9 of 20 Pseudo Genes (incomplete) :: 9 of 20 Pseudo Genes (internal stop) :: 3 of 20 Pseudo Genes (multiple problems) :: 1 of 20 CRISPR Arrays :: 1 ##Genome-Annotation-Data-END## FEATURES Location/Qualifiers source 1..23 /organism="Listeria monocytogenes" /mol_type="genomic DNA" /strain="PNUSAL011489" /db_xref="taxon:1639" /collection_date="2021" WGS ABCGTD010000001-ABCGTD010000023 //