PRIMER_TASK=check_primers
PRIMER_PICK_ANYWAY=1
PRIMER_NUM_RETURN=1
PRIMER_EXPLAIN_FLAG=1
PRIMER_MAX_NS_ACCEPTED=100
PRIMER_MAX_SIZE=35
PRIMER_MIN_SIZE=14
PRIMER_PRODUCT_SIZE_RANGE=60-1000
PRIMER_PICK_LEFT_PRIMER=1
PRIMER_PICK_RIGHT_PRIMER=1
PRIMER_PICK_INTERNAL_OLIGO=0
PRIMER_SECONDARY_STRUCTURE_ALIGNMENT=1
SEQUENCE_ID=Overhang01
SEQUENCE_TEMPLATE=GACATACGATACTTTGCTTTCAATCATGTGGGTGAAACGACTAAACCTTACGACTACTTAAAGTGGACTGGTAGTCTTTTTAAAGGTAC
SEQUENCE_PRIMER=TGCTTTCAATCATGTGGGTGA
SEQUENCE_OVERHANG_LEFT=TCACCCAC
SEQUENCE_PRIMER_REVCOMP=GACTACCAGTCCACTTTAAG
SEQUENCE_OVERHANG_RIGHT=TAAGGATTT
=