PRIMER_TM_FORMULA=1 PRIMER_SALT_CORRECTIONS=1 PRIMER_DNA_CONC=50 PRIMER_SALT_MONOVALENT=50 PRIMER_SALT_DIVALENT=1.5 PRIMER_DNTP_CONC=0.6 PRIMER_DMSO_CONC=0 PRIMER_DMSO_FACTOR=0.6 PRIMER_FORMAMIDE_CONC=0