[UGENE-4123] Create "Get unmapped reads" sample workflow Created: 04/Mar/15  Updated: 31/Oct/18  Resolved: 09/Mar/15

Status: Closed
Project: UGENE
Component/s: Workflow
Affects Version/s: 1.16
Fix Version/s: 1.16.1

Type: Task Priority: Major
Reporter: Olga Golosova Assignee: Yura Vaskin
Resolution: Fixed  
Labels: NGS, request
Remaining Estimate: Not Specified
Time Spent: Not Specified
Original Estimate: Not Specified

Attachments: PNG File samtools_flags.png    
Assigned Tester: Denis Kandrov
Tests Type: Functional/Unit
Affect Type: Userdefined

 Description   

See http://ugene.unipro.ru/forum/YaBB.pl?num=1425320889/2#2

It may be required to analyze unmapped reads:

using unmatched reads I was able to perform de novo sequencing and I have some huge fragments of DNA (different set of plasmids, still analyse it)

So, a separate workflow sample can be created for this.

For example, "Filter BAM/SAM files using samtools view" may be used for this, i.e. not to filter reads by a flag, but filter all other reads that do not have the flag. An additional parameter must be added for the worker for doing this.



 Comments   
Comment by Denis Kandrov [ 18/Mar/15 ]

Checked.

Comment by Yuliya Algaer [ 31/Mar/15 ]

Reviewed.

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