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  1. UGENE
  2. UGENE-2639

Tuxedo pipeline doesn't work on data drosophila data

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    Details

    • Type: Bug
    • Status: Closed
    • Priority: Critical
    • Resolution: Fixed
    • Affects Version/s: 1.13
    • Fix Version/s: 1.13.1, 1.14
    • Component/s: Workflow
    • Labels:
      None
    • Environment:

      Mac OS X

    • Sprint:
      DEV-2014/1/16, TEST-2014/01/23, TEST-2014/01/30, TEST-2014/02/06, TEST-2014/02/13, TEST-20/02/2014, DEV-06/03/2014, TEST-13/03/2014, TEST-27/03/2014
    • Affect Type:
      Userdefined

      Description

      An error occurs when Tuxedo pipeline is run on data specified in the Tuxedo article:

      • Input paired-end reads datasets:
        1) GSM794483_C1_R1_1.fq.gz and GSM794483_C1_R1_2.fq.gz (i.e. C1_R1)
        2) GSM794484_C1_R2_1.fq.gz and GSM794484_C1_R2_2.fq.gz (i.e. C1_R2)
        3) GSM794485_C1_R3_1.fq.gz and GSM794485_C1_R3_2.fq.gz (i.e. C1_R3)
        4) GSM794486_C2_R1_1.fq.gz and GSM794486_C2_R1_2.fq.gz (i.e. C2_R1)
        5) GSM794487_C2_R2_1.fq.gz and GSM794487_C2_R2_2.fq.gz (i.e. C2_R2)
        6) GSM794488_C2_R3_1.fq.gz and GSM794488_C2_R3_2.fq.gz (i.e. C2_R3)
      • Split datasets to samples: first three datasets to the first sample, last three datasets to the second sample.
      • Bowtie index of drosophila_melanogaster: "BDGP5.25/Sequence/Bowtie2Index/genome.*" files from the iGenome package
      • Known transcript file: file "BDGP5.25/Annotation/Archives/archive-2013-03-06-11-13-59/Genes/genes.gtf"

      UGENE version used 4 threads.

      Note: the data can found on the UGENE local file server in "data/tuxedo_paper_data" directory.

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            Assignee:
            ggrekhov German Grekhov
            Reporter:
            oigl Olga Golosova
            Assigned Tester:
            Aleksey Tiunov [X] (Inactive)
            Watchers:
            2 Start watching this issue

              Dates

              Created:
              Updated:
              Resolved:

                Time Tracking

                Estimated:
                Original Estimate - 2 hours
                2h
                Remaining:
                Remaining Estimate - 2 hours
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