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  1. UGENE
  2. UGENE-7636

Primer details for "In Silico PCR" give different results

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    Details

    • Type: Bug
    • Status: Closed
    • Priority: Major
    • Resolution: Cannot Reproduce
    • Affects Version/s: 39, 38.1, 40, 41, 40.1, 42
    • Fix Version/s: None
    • Component/s: Basic-Nucl, seq
    • Labels:
    • Tests Type:
      Functional/Unit
    • Affect Type:
      Userdefined

      Description

      Reproduced in version 38.1, but the bug may have existed before.

      1. Open _common_data/pcr_primer_design/gfp.fa.
      2. Open the OP "In Silico PCR" tab.
      3. Fill in:
        1. Forward primer: GAACTATATTTTTCAATGGGCACAAATTTTCTGTC;
        2. Reverse primer: TACTCATTTCCTCTTCTTGAAAAGTGACCTCAACAGGGTTAAGAACAACTTAATCTACCACTACAATTACCCGTGTTTAAAAGACAGTCACCTCTCCCACTTCCACTACGTTGTATGCCTTTTGAATGGGAATTTAAATAAACGTGATGACCTTTTGATGGACAAGGTACCGGTTGTGAACAGTGATGAAAGCCAAT;
        3. 3' perfect match: 0.
      4. Repeat the following steps many times (~20 or more):
        1. Click "Show primers details". The "Primer Details" dialog appears;
        2. Enlarge the dialog to see the heterodimer characteristics (Delta G and Base Pairs);
        3. Expected: the hetero-dimer and its characteristics are always the same across runs, e. g. "Delta G=-7.4, Base Pairs=5",
          Current state: hetero-dimers may differ, for example 
        4. Close the dialog (you can use Alt+F4) and repeat the steps until the bug appears.

      Perhaps repetitive steps need to be done quickly.

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            Assignee:
            dsukhomlinov Dmitrii Sukhomlinov
            Reporter:
            biserova Evelina
            Watchers:
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              Dates

              Created:
              Updated:
              Resolved: