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  1. UGENE
  2. UGENE-7825

Primer3 has shift in the result primers

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    Details

    • Type: Bug
    • Status: Closed
    • Priority: Major
    • Resolution: Fixed
    • Affects Version/s: 46
    • Fix Version/s: 47
    • Component/s: None
    • Labels:

      Description

      Scenario:

      1. Open the attached sequence.
      2. Double click the annotation.
      3. Open Primer3 dialog
      4. Set "pick_discriminative_primers" task
      5. Set Target region to 36,163 (annotated region)
      6. Set region to whole sequence
      7. Go to Advance settings tab and click "Pick anyway"
      8. Click "Pick"
        Expected: primers frame the annotation
        Current: primers have one character shift

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            Assignee:
            yalgaer Yuliya Algaer
            Reporter:
            dsukhomlinov Dmitrii Sukhomlinov
            Assigned Tester:
            Svetlana Samoilenko
            Watchers:
            2 Start watching this issue

              Dates

              Created:
              Updated:
              Resolved: