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  1. UGENE
  2. UGENE-7926

Wrong Base Pairs value for self/hetero-dimers

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    Details

    • Type: Bug
    • Status: Closed
    • Priority: Major
    • Resolution: Fixed
    • Affects Version/s: None
    • Fix Version/s: 49
    • Component/s: None
    • Labels:
      None
    • Epic Link:
    • Sprint:
      DEV-49-3, DEV-49-4
    • Affect Type:
      Userdefined

      Description

      1. Open human_T1.fa.
      2. Open "In silico pcr" tab
      3. Forward primer - GTGTAAAATATGCAGAAGAGCATCTAACA
        Reverse primer - GCTCAAAAACCCAATTCTAAGGGG
      4. Click "Show primer details".
      5. Look at the first "Self-dimer".
        Current

        Important, that Base Pair: 5
        Expected: (the same dimer, but from OligoAnalyzer)

        Base Pairs: 4

       

      PS, I am pretty sure, that I have already faced and fixed this in Pcr Primer Design, but we did not merge this branch. Check this - I am sure, this is the working fix.

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              Assignee:
              dsukhomlinov Dmitrii Sukhomlinov
              Reporter:
              dsukhomlinov Dmitrii Sukhomlinov
              Assigned Tester:
              Svetlana Samoilenko
              Watchers:
              2 Start watching this issue

                Dates

                Created:
                Updated:
                Resolved: