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  1. UGENE
  2. UGENE-856

Primer 3 option: span exon-intron boundaries

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    Details

    • Type: Improvement
    • Status: Closed
    • Priority: Major
    • Resolution: Fixed
    • Affects Version/s: None
    • Fix Version/s: 1.11
    • Component/s: Basic-Nucl
    • Labels:
      None
    • Affect Type:
      Userdefined

      Description

      The option should allow to provide an mRNA sequence (or database identifier) to locate exon-intron boundaries within the sequence. After that the user should be able to choose what exons to amplify. If the product of primer doesn't span the exon region than it should be excluded from results.

      The similar features are available in:
      1) PerlPrimer (http://perlprimer.sourceforge.net/index.html)
      2) PrimerBLAST (http://www.ncbi.nlm.nih.gov/tools/primer-blast/)

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              Assignee:
              kokonech Konstantin Okonechnikov (Inactive)
              Reporter:
              kokonech Konstantin Okonechnikov (Inactive)
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                Dates

                Created:
                Updated:
                Resolved: