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Type: Bug
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Status: Closed
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Priority: Critical
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Resolution: Fixed
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Affects Version/s: 1.31.1
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Fix Version/s: 33
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Component/s: Basic-Nucl
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Story Points:2
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Sprint:DEV-33-1, DEV-33-2
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Affect Type:Userdefined
Precondition:
- There is a nucleotide sequence (e.g. "murine.fa").
- There is a BLAST+ database with amino acid sequences homologous to the translated nucleotide sequence (e.g. unpacked "murinedb2" - note that it was built from amino acid translation of "murine" reverse-complement sequence).
Scenario:
- Open the nucleotide sequence.
- Select "Actions > Analyze > Query with local BLAST+".
- In the appeared dialog set "Select search" to "blastx", specify the database, click "Search".
Expected result: "blast result" annotation, located in region 2..5833, is located on the reverse-complement strand.
Current result: all "blast result" annotations are located on the direct strand.
For details, see the attached "murine.xml" file also. The original BLAST tool return an xml file. For the annotation specified above in the "Expected result", "Hsp_query-frame" is negative ("-1"). Currently, this is not taken into account.