Primer-BLAST, included in UGENE-7921, could BLAST only primers, which are produced by Primer3. Sometimes, user could have primers, which he's got from some external sources:
In this case, primer pair is not defined as a fit primer pair to be BLASTed.
The best solution here is to select primers you want to blast, click some button and make copyes of them in a "top_primer" annotations table (default annotation table for primer pairs).
Scenario:
- Open _common_data\primer3\custom_primers.gb.
- Select "primer1" and "primer2" annotations.
- Click right mouse button -> Edit -> Transform into a primer pair.
Expected: new primer pair has appeared.
- relates to
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UGENE-8046 Hot keys Shift+T does not transform into a primer pair
- Open
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UGENE-8041 Help: Add in some paragraph a description of new feature: "Transform into a primer pair"
- Closed