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  1. UGENE
  2. UGENE-6649

Invalid extracted PCR product when a primer goes through the circular sequence start coordinate

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    Details

    • Type: Bug
    • Status: Closed
    • Priority: Major
    • Resolution: Fixed
    • Affects Version/s: 33
    • Fix Version/s: 34
    • Component/s: Basic-Nucl
    • Labels:
    • Story Points:
      2
    • Sprint:
      DEV-34-6, DEV-34-7, DEV-34-8, DEV-34-9
    • Affect Type:
      Userdefined

      Description

      See issue 2 from here: http://ugene.net/forum/YaBB.pl?num=1573198816/3#3.

      Scenario:

      1. Open the attached sequence "pET-24.gb".
        Expected result: the sequence is circular (icon for the sequence object in the Project View is circular). The Sequence View with the Circular Vies is shown.
      2. Open the "In Silico PCR" tab on the options panel.
      3. Set:
        • "Forwad primer" to "GCTCTCCCTTATGCGACTCC".
        • "Reverse primer" to "GCGTCCCATTCGCCAATCC".
      4. Click the "Find product anyway" button.
        Expected result: length of the extracted region is 642 bp.
      5. Click the "Extract product(s)" button.
        Expected result  the extracted sequence is 642 bp long.
        Actual result:  the extracted sequence is 39 bp long, it is made of primers joined together.

        Attachments

        1. pET-24.gb
          12 kB
          Olga Golosova

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              Assignee:
              kir Kirill Rasputin
              Reporter:
              s.samoilenko Svetlana Samoilenko
              Assigned Tester:
              Svetlana Samoilenko
              Watchers:
              2 Start watching this issue

                Dates

                Created:
                Updated:
                Resolved: